Título:	Insights into the Melipona scutellaris (Hymenoptera, Apidae, Meliponini) fat body transcriptome
Autor(es):	Sousa, Cristina Soares de Serrão, José Eduardo Bonetti, Ana Maria Amaral, Isabel Marques Rodrigues Kerr, Warwick Estevam Maranhão, Andréa Queiroz Ueira-Vieira, Carlos
Assunto:	Genética Abelha Melipona Gordura
Data de publicação:	2013
Editora:	Sociedade Brasileira de Genética
Referência:	SOUSA, Cristina Soares de et al. Insights into the Melipona scutellaris (Hymenoptera, Apidae, Meliponini) fat body transcriptome. Genetics and Molecular Biology, São Paulo, v. 36, n. 2, p. 292-297, 2013. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200022&lng=pt&nrm=iso>. Acesso em: 14 dez. 2016. http://dx.doi.org/10.1590/S1415-47572013000200022.
Abstract:	The insect fat body is a multifunctional organ analogous to the vertebrate liver. The fat body is involved in the metabolism of juvenile hormone, regulation of environmental stress, production of immunity regulator-like proteins in cells and protein storage. However, very little is known about the molecular mechanisms involved in fat body physiology in stingless bees. In this study, we analyzed the transcriptome of the fat body from the stingless bee Melipona scutellaris. In silico analysis of a set of cDNA library sequences yielded 1728 expressed sequence tags (ESTs) and 997 high-quality sequences that were assembled into 29 contigs and 117 singlets. The BLAST X tool showed that 86% of the ESTs shared similarity with Apis mellifera (honeybee) genes. The M. scutellaris fat body ESTs encoded proteins with roles in numerous physiological processes, including anti-oxidation, phosphorylation, metabolism, detoxification, transmembrane transport, intracellular transport, cell proliferation, protein hydrolysis and protein synthesis. This is the first report to describe a transcriptomic analysis of specific organs of M. scutellaris. Our findings provide new insights into the physiological role of the fat body in stingless bees.
Licença:	Genetics and Molecular Biology - License information: This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Fonte: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200022&lng=pt&nrm=iso. Acesso em: 14 dez. 2016.
DOI:	http://dx.doi.org/10.1590/S1415-47572013000200022
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