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dc.contributor.authorMaranhão, Andrea Queiroz-
dc.contributor.authorCosta, Maria Beatriz Walter-
dc.contributor.authorGuedes, Leonardo-
dc.contributor.authorMoraes-Vieira, Pedro Manoel-
dc.contributor.authorRaiol, Tainá-
dc.contributor.authorBrígido, Marcelo de Macedo-
dc.date.accessioned2016-12-21T14:41:28Z-
dc.date.available2016-12-21T14:41:28Z-
dc.date.issued2013-
dc.identifier.citationMARANHÃO, Andrea Queiroz et al. A mouse variable gene fragment binds to DNA independently of the BCR context: a possible role for immature b-cell repertoire establishment. Plos One, v. 8, n. 9, p. e72625, 2013. Disponível em: <http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0072625 >. Acesso em: 14 dez. 2016. http://dx.doi.org/10.1371/journal.pone.0072625en
dc.identifier.urihttp://repositorio.unb.br/handle/10482/22024-
dc.description.abstractB-cell maturation occurs in several steps and requires constant stimulus for its continuing development. From the emergence of the pre-B-cell receptor, signal transduction stimulates and supports B-cell development. Current viewpoints indicate that both positive selection pressure for autoantigens and tonic signaling constitutively stimulate B-cell maturation. In this work, we tested for the presence of a putative DNA binding site in a variable gene segment in a germline configuration, independently of VDJ recombination. After a survey of the public antibody databases, we chose a single mouse heavy variable gene segment that is highly represented in anti-nucleic acid antibodies and tested it for ssDNA binding. A phage display approach was used to search for intrinsic binding to oligo deoxythymidine. The results revealed that binding to an antigen can be influenced by the use of a specific DNA binding V gene segment. Our data support the idea that some variable genes have intrinsic reactivity towards specific types of endogenous autoantigens, and this property may contribute to the establishment of the immature B-cell repertoire.en
dc.language.isoInglêsen
dc.publisherPlos Oneen
dc.rightsAcesso Abertoen
dc.titleA mouse variable gene fragment binds to DNA independently of the BCR context : a possible role for immature b-cell repertoire establishmenten
dc.typeArtigoen
dc.subject.keywordReações antígeno-anticorpoen
dc.subject.keywordMaturação celularen
dc.rights.licensePlos One - Copyright: 2013 Maranhão et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Fonte: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0072625. Acesso em: 14 dez. 2016.en
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0072625en
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