Skip navigation
Use este identificador para citar ou linkar para este item: http://repositorio.unb.br/handle/10482/23745
Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
ARTIGO_CytotoxicityAnalysis of Three.PDF534,96 kBAdobe PDFVisualizar/Abrir
Registro completo de metadados
Campo DCValorIdioma
dc.contributor.authorCorrêa, Roberto Franco Teixeira-
dc.contributor.authorAraújo, Daniel Mendes Pereira Ardisson-
dc.contributor.authorMonnerat, Rose Gomes-
dc.contributor.authorRibeiro, Bergmann Morais-
dc.date.accessioned2017-06-26T15:00:12Z-
dc.date.available2017-06-26T15:00:12Z-
dc.date.issued2012-09-21-
dc.identifier.citationCORRÊA, Roberto Franco Teixeira et al. Cytotoxicity analysis of three Bacillus thuringiensis Subsp. israelensis d-Endotoxins towards insect and mammalian cells. Plos One, v. 7, n. 9, Article e46121, 21 set. 2012. Disponível em: <http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0046121>. Acesso em: 13 jun. 2017. doi: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0046121.pt_BR
dc.identifier.urihttp://repositorio.unb.br/handle/10482/23745-
dc.language.isoInglêspt_BR
dc.publisherPlos Onept_BR
dc.rightsAcesso Abertopt_BR
dc.titleCytotoxicity analysis of three Bacillus thuringiensis Subsp. israelensis d-Endotoxins towards insect and mammalian cellspt_BR
dc.typeArtigopt_BR
dc.subject.keywordToxinaspt_BR
dc.subject.keywordInsetopt_BR
dc.subject.keywordMamíferopt_BR
dc.subject.keywordProteínaspt_BR
dc.rights.licenseCopyright: 2012 Teixeira Corrêa et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.pt_BR
dc.identifier.doihttps://dx.doi.org/10.1371/journal.pone.0046121pt_BR
dc.description.abstract1Three members of the d-endotoxin group of toxins expressed by Bacillus thuringiensis subsp. israelensis, Cyt2Ba, Cry4Aa and Cry11A, were individually expressed in recombinant acrystalliferous B. thuringiensis strains for in vitro evaluation of their toxic activities against insect and mammalian cell lines. Both Cry4Aa and Cry11A toxins, activated with either trypsin or Spodoptera frugiperda gastric juice (GJ), resulted in different cleavage patterns for the activated toxins as seen by SDS-PAGE. The GJ-processed proteins were not cytotoxic to insect cell cultures. On the other hand, the combination of the trypsinactivated Cry4Aa and Cry11A toxins yielded the highest levels of cytotoxicity to all insect cells tested. The combination of activated Cyt2Ba and Cry11A also showed higher toxic activity than that of toxins activated individually. When activated Cry4Aa, Cry11A and Cyt2Ba were used simultaneously in the same assay a decrease in toxic activity was observed in all insect cells tested. No toxic effect was observed for the trypsin-activated Cry toxins in mammalian cells, but activated Cyt2Ba was toxic to human breast cancer cells (MCF-7) when tested at 20 mg/mL.pt_BR
Aparece nas coleções:Artigos publicados em periódicos e afins

Mostrar registro simples do item Visualizar estatísticas



Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.