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dc.contributor.authorTavares, Aldo Henrique Fonseca Pacheco-
dc.contributor.authorDerengowski, Lorena da Silveira-
dc.contributor.authorFerreira, Karen S.-
dc.contributor.authorSilva, Simoneide Sousa-
dc.contributor.authorMacedo, Cláudia-
dc.contributor.authorBocca, Anamélia Lorenzetti-
dc.contributor.authorPassos, Geraldo A-
dc.contributor.authorAlmeida, Sandro R.-
dc.contributor.authorIldinete Silva, Pereira-
dc.date.accessioned2017-07-17T12:42:44Z-
dc.date.available2017-07-17T12:42:44Z-
dc.date.issued2012-01-03-
dc.identifier.citationTAVARES, Aldo Henrique Fonseca Pacheco et al. Murine dendritic cells transcriptional modulation upon Paracoccidioides brasiliensis infection. Plos One Neglected Tropical Diseases, v. 6, n. 1, Article e1459, 3 jan. 2012. Disponível em: <http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0001459>. Acesso em: 12 jun. 2017. doi: http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0001459.pt_BR
dc.identifier.urihttp://repositorio.unb.br/handle/10482/23837-
dc.language.isoInglêspt_BR
dc.publisherPlos One Neglected Tropical Diseasespt_BR
dc.rightsAcesso Abertopt_BR
dc.titleMurine dendritic cells transcriptional modulation upon Paracoccidioides brasiliensis infectionpt_BR
dc.typeArtigopt_BR
dc.subject.keywordMicoses - Paracoccidioides brasiliensispt_BR
dc.subject.keywordCélulaspt_BR
dc.subject.keywordMedula ósseapt_BR
dc.rights.license© 2012 Tavares et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.pt_BR
dc.identifier.doihttps://dx.doi.org/10.1371/journal.pntd.0001459pt_BR
dc.description.abstract1Limited information is available regarding the modulation of genes involved in the innate host response to Paracoccidioides brasiliensis, the etiologic agent of paracoccidioidomycosis. Therefore, we sought to characterize, for the first time, the transcriptional profile of murine bone marrow-derived dendritic cells (DCs) at an early stage following their initial interaction with P. brasiliensis. DCs connect innate and adaptive immunity by recognizing invading pathogens and determining the type of effector T-cell that mediates an immune response. Gene expression profiles were analyzed using microarray and validated using real-time RT-PCR and protein secretion studies. A total of 299 genes were differentially expressed, many of which are involved in immunity, signal transduction, transcription and apoptosis. Genes encoding the cytokines IL-12 and TNF-a, along with the chemokines CCL22, CCL27 and CXCL10, were up-regulated, suggesting that P. brasiliensis induces a potent proinflammatory response in DCs. In contrast, pattern recognition receptor (PRR)-encoding genes, particularly those related to Toll-like receptors, were down-regulated or unchanged. This result prompted us to evaluate the expression profiles of dectin-1 and mannose receptor, two other important fungal PRRs that were not included in the microarray target cDNA sequences. Unlike the mannose receptor, the dectin-1 receptor gene was significantly induced, suggesting that this bglucan receptor participates in the recognition of P. brasiliensis. We also used a receptor inhibition assay to evaluate the roles of these receptors in coordinating the expression of several immune-related genes in DCs upon fungal exposure. Altogether, our results provide an initial characterization of early host responses to P. brasiliensis and a basis for better understanding the infectious process of this important neglected pathogen.pt_BR
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