Título:	A set of multiplex panels of microsatellite markers for rapid molecular characterization of rice accessions
Autor(es):	Pessoa Filho, Marco Beló, André Alcochete, António A. N. Rangel, Paulo H. N. Ferreira, Márcio Elias
Assunto:	Microssatélites Arroz Germoplasma vegetal
Data de publicação:	21-Mai-2007
Editora:	BioMed Central
Referência:	PESSOA FILHO, Marco et al. A set of multiplex panels of microsatellite markers for rapid molecular characterization of rice accessions. BMC Plant Biology, v. 7, Article 23, 21 mai. 2007. Disponível em: <https://bmcplantbiol.biomedcentral.com/articles/10.1186/1471-2229-7-23>. Acesso em: 19 jun. 2017. doi: https://bmcplantbiol.biomedcentral.com/articles/10.1186/1471-2229-7-23.
Abstract:	Background: This study aimed to analyze the efficiency of three new microsatellite multiplex panels, which were designed to evaluate a total of 16 loci of the rice genome, based on single PCR reactions of each panel. A sample of 548 accessions of traditional upland rice landraces collected in Brazil in the last 25 years was genotyped, a database of allelic frequencies was established, estimates of genetic parameters were performed and analysis of genetic structure of the collection was developed. Results: The three panels yielded a combined matching probability of 6.4 × 10-21, polymorphism information content (PIC) of 0.637, and a combined power of exclusion greater than 99.99%. A few samples presented a genetic background of indica rice. The 16 SSR loci produced a total of 229 alleles. Gene diversity values averaged 0.667, and PIC values averaged 0.637. Genetic structure analysis of the collection using a Bayesian approach detected three possible major clusters, with an overall FST value of 0.177. Important inputs on the knowledge about upland rice germplasm differentiations which happened in Brazil in the last few centuries were also achieved and are discussed. Conclusion: The three multiplex panels described here represent a powerful tool for rice genetic analysis, offering a rapid and efficient option for rice germplasm characterization. The data gathered demonstrates the feasibility of genotyping extensive germplasm collections using panels of multiplexed microsatellite markers. It contributes to the advancement of research on large scale characterization and management of germplasm banks, as well as identification, protection and assessments of genetic relationship of rice germplasm.
Licença:	© 2007 Pessoa-Filho et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
DOI:	https://dx.doi.org/10.1186/1471-2229-7-23
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