Title:	A saturated SSR/DArT linkage map of Musa acuminata addressing genome rearrangements among bananas
Authors:	Hippolyte, Isabelle Bakry, Frederic Seguin, Marc Gardes, Laetitia Rivallan, Ronan Risterucci, Ange Marie Jenny, Christophe Perrier, Xavier Carreel, Françoise Argout, Xavier Piffanelli, Pietro Khan, Imtiaz A. Miller, Robert Neil Gerard Pappas Júnior, Georgios Joannis Mbéguié-A-Mbéguié, Didier Matsumoto, Takashi Bernardinis, Veronique de Huttner, Eric Kilian, Andrzej Baurens, Franc Christophe D'Hont, Angélique Cote, François Courtois, Brigitte Glaszmann, Jean Christophe
Assunto::	Banana - cultivo Banana - genética
Issue Date:	13-Apr-2010
Publisher:	BioMed Central
Citation:	HIPPOLYTE, Isabelle et al. A saturated SSR/DArT linkage map of Musa acuminata addressing genome rearrangements among bananas. BMC Plant Biology, v. 10, Article 65, 13 abr. 2010. Disponível em: <https://bmcplantbiol.biomedcentral.com/articles/10.1186/1471-2229-10-65>. Acesso em: 26 jun. 2017. doi: 10.1186/1471-2229-10-65.
Abstract:	Background: The genus Musa is a large species complex which includes cultivars at diploid and triploid levels. These sterile and vegetatively propagated cultivars are based on the A genome from Musa acuminata, exclusively for sweet bananas such as Cavendish, or associated with the B genome (Musa balbisiana) in cooking bananas such as Plantain varieties. In M. acuminata cultivars, structural heterozygosity is thought to be one of the main causes of sterility, which is essential for obtaining seedless fruits but hampers breeding. Only partial genetic maps are presently available due to chromosomal rearrangements within the parents of the mapping populations. This causes large segregation distortions inducing pseudo-linkages and difficulties in ordering markers in the linkage groups. The present study aims at producing a saturated linkage map of M. acuminata, taking into account hypotheses on the structural heterozygosity of the parents. Results: An F1 progeny of 180 individuals was obtained from a cross between two genetically distant accessions of M. acuminata, 'Borneo' and 'Pisang Lilin' (P. Lilin). Based on the gametic recombination of each parent, two parental maps composed of SSR and DArT markers were established. A significant proportion of the markers (21.7%) deviated (p < 0.05) from the expected Mendelian ratios. These skewed markers were distributed in different linkage groups for each parent. To solve some complex ordering of the markers on linkage groups, we associated tools such as tree-like graphic representations, recombination frequency statistics and cytogenetical studies to identify structural rearrangements and build parsimonious linkage group order. An illustration of such an approach is given for the P. Lilin parent. Conclusions: We propose a synthetic map with 11 linkage groups containing 489 markers (167 SSRs and 322 DArTs) covering 1197 cM. This first saturated map is proposed as a "reference Musa map" for further analyses. We also propose two complete parental maps with interpretations of structural rearrangements localized on the linkage groups. The structural heterozygosity in P. Lilin is hypothesized to result from a duplication likely accompanied by an inversion on another chromosome. This paper also illustrates a methodological approach, transferable to other species, to investigate the mapping of structural rearrangements and determine their consequences on marker segregation.
Licença::	© 2010 Hippolyte et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
DOI:	https://dx.doi.org/10.1186/1471-2229-10-65
Appears in Collections:	Artigos publicados em periódicos e afins

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