Título:	Shedding some light over the floral metabolism by Arum Lily (Zantedeschia aethiopica) Spathe de novo transcriptome assembly
Autor(es):	Cândido, Elizabete de Souza Fernandes, Gabriel da Rocha Alencar, Sérgio Amorim de Cardoso, Marlon Henrique e Silva Lima, Stella Maris de Freitas Miranda, Vívian de Jesus Porto, William Farias Nolasco, Diego Oliveira Oliveira Júnior, Nelson Gomes de Barbosa, Aulus Estevão Anjos de Deus Pogue, Robert Edward Rezende, Taia Maria Berto Dias, Simoni Campos Franco, Octávio Luiz
Assunto:	Copo de leite Bacterioses Proteínas
Data de publicação:	10-Mar-2014
Editora:	Plos One
Referência:	CÂNDIDO, Elizabete de Souza et al. Shedding some light over the floral metabolism by Arum Lily (Zantedeschia aethiopica) Spathe de novo transcriptome assembly. Plos One, v. 9, n. 3, Article e90487, 10 mar. 2014. Disponível em: <http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0090487>. Acesso em: 22 jun. 2017. doi: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0090487.
Abstract:	Zantedeschia aethiopica is an evergreen perennial plant cultivated worldwide and commonly used for ornamental and medicinal purposes including the treatment of bacterial infections. However, the current understanding of molecular and physiological mechanisms in this plant is limited, in comparison to other non-model plants. In order to improve understanding of the biology of this botanical species, RNA-Seq technology was used for transcriptome assembly and characterization. Following Z. aethiopica spathe tissue RNA extraction, high-throughput RNA sequencing was performed with the aim of obtaining both abundant and rare transcript data. Functional profiling based on KEGG Orthology (KO) analysis highlighted contigs that were involved predominantly in genetic information (37%) and metabolism (34%) processes. Predicted proteins involved in the plant circadian system, hormone signal transduction, secondary metabolism and basal immunity are described here. In silico screening of the transcriptome data set for antimicrobial peptide (AMP) – encoding sequences was also carried out and three lipid transfer proteins (LTP) were identified as potential AMPs involved in plant defense. Spathe predicted protein maps were drawn, and suggested that major plant efforts are expended in guaranteeing the maintenance of cell homeostasis, characterized by high investment in carbohydrate, amino acid and energy metabolism as well as in genetic information.
Licença:	Copyright: 2014 Cândido et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DOI:	https://dx.doi.org/10.1371/journal.pone.0090487
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