Título:	Non-disulfide-Bridge peptide 5.5 from the Scorpion Hadrurus gertschi Inhibits the growth of mycobacterium abscessus subsp. massiliense
Autor(es):	Trentini, Monalisa Martins Neves, Rogério Coutinho das Santos, Bruno de Paula Oliveira Silva, Roosevelt Alves da Souza, Adolfo Carlos Mortari, Márcia Renata Schwartz, Elisabeth Nogueira Ferroni Kipnis, André Kipnis, Ana Paula Junqueira
Assunto:	Infecção Microorganismos Escorpião - veneno Agentes antibacterianos Peptídeos
Data de publicação:	22-Fev-2017
Editora:	Frontiers
Referência:	TRENTINI, Monalisa Martins et al. Non-disulfide-Bridge peptide 5.5 from the Scorpion Hadrurus gertschi Inhibits the growth of mycobacterium abscessus subsp. massiliense. Frontiers in Microbiology, v. 8, Article 273, 22 fev. 2017. Disponível em: <http://journal.frontiersin.org/article/10.3389/fmicb.2017.00273/full>. Acesso em: 27 jul. 2017. doi: https://doi.org/10.3389/fmicb.2017.00273.
Abstract:	Multi-drug resistant microorganisms have been a growing concern during the last decades due to their contribution in mortality rates worldwide. Antimicrobial peptides (AMPs) are broad spectrum antimicrobial agents that display potent microbicidal activity against a wide range of microorganisms. AMPs generally have a rapid mode of action that reduces the risk of resistance developing among pathogens. In this study, an AMP derived from scorpion venom, NDBP-5.5, was evaluated against Mycobacterium abscessus subsp. massiliense, a rapidly growing and emerging pathogen associated with healthcare infections. The minimal bactericidal concentration of NDBP-5.5, AMP quantity necessary to stop bacteria visible growth, against M. abscessus subsp. massiliense was 200 μM, a concentration that did not induce hemolysis of human red blood cells. The therapeutic index was 3.05 indicating a drug with low toxicity and therefore good clinical potential. Treatment of infected macrophages with NDBP-5.5 or clarithromycin presented similar results, reducing the bacterial load. M. abscessus subsp. massiliense-infected animals showed a decrease in the bacterial load of up to 70% when treated with NDBP-5.5. These results revealed the effective microbicidal activity of NDBP-5.5 against Mycobacterium, indicating its potential as an antimycobacterial agent.
Licença:	Copyright © 2017 Trentini, das Neves, Santos, DaSilva, Souza, Mortari, Schwartz, Kipnis and Junqueira-Kipnis. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
DOI:	https://doi.org/10.3389/fmicb.2017.00273
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