Título:	Effects of pneumoperitoneum with carbon dioxide and helium on renal function and morphology in rats
Autor(es):	Freitas, Pedro Felipe Silva de Durães, Leonardo de Castro Carvalho, Felipe Augusto Neves Oliveira de Duarte, Sérgio Andurte Carvalho Carneiro, Fabiana Pirani Sousa, João Batista de
Assunto:	Cirurgia Laparoscopia Pneumoperitônio Dióxido de carbono Rins Ratos
Data de publicação:	Jul-2013
Editora:	Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Referência:	FREITAS, Pedro Felipe Silva de et al. Effects of pneumoperitoneum with carbon dioxide and helium on renal function and morphology in rats. Acta Cirúrgica Brasileira, São Paulo, v. 28, n. 7, p. 494-498, jul. 2013. DOI: http://dx.doi.org/10.1590/S0102-86502013000700003. Disponível: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013000700003&lng=en&nrm=iso. Acesso em: 04 dez. 2020.
Abstract:	PURPOSE: To evaluate the effects of pneumoperitoneum with carbon dioxide and helium on renal function and morphology in a rat model. METHODS: Twenty four rats were randomized into three groups (n=8): gasless insufflation ('open', Pressure=0 mmHg), carbon dioxide pneumoperitoneum at 12 mmHg, and helium pneumoperitoneum at 12 mmHg; all lasting 90 minutes.. A cystostomy was performed and the bladder was emptied. At the end of the experiment, the urine produced, a blood sample and the left kidney of each animal were collected. The following variables were obtained: serum sodium, potassium, urea and creatinine, urine volume and creatinine. The creatinine clearance was estimated for each animal. The kidneys were stained with hematoxylin and eosin (HE) and evaluated by a pathologist blinded to the groups. RESULTS: The CO2 and Helium groups did not differ in the variables evaluated. Both developed oliguria (p<0.001 vs. gasless). The CO2 group presented hyperkalemia compared to gasless (p=0.05), which did not attain significance in the helium group. Histopathological analysis revealed mild hydropic degeneration and congestion in the three groups, with no significant difference among them. CONCLUSIONS: The type of gas resulted in no difference in the variables of renal function and morphology assessed. The increase in serum potassium was only observed with CO2 insufflation suggests a combined effect of elevated intra-abdominal pressure and metabolic effects of pneumoperitoneum.
Unidade Acadêmica:	Faculdade de Medicina (FMD)
Licença:	Acta Cirúrgica Brasileira - All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License (CC BY NC 4.0). Fonte: https://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013000700003&lng=en&tlng=en. Acesso em: 04 dez. 2020.
DOI:	https://dx.doi.org/10.1590/S0102-86502013000700003
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