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dc.contributor.authorLamounier, Thaís Alves da Costapt_BR
dc.contributor.authorOliveira, Layssa Miranda dept_BR
dc.contributor.authorCamargo, Brenda Rabello dept_BR
dc.contributor.authorRodrigues, Kelly Barretopt_BR
dc.contributor.authorNoronha, Eliane Ferreirapt_BR
dc.contributor.authorRibeiro, Bergmann Moraispt_BR
dc.contributor.authorNagata, Tatsuyapt_BR
dc.date.accessioned2017-12-07T05:13:27Z-
dc.date.available2017-12-07T05:13:27Z-
dc.date.issued2015-10pt_BR
dc.identifier.citationLAMOUNIER, Thais Alves da Costa et al. Production of Brazilian human norovirus VLPs and comparison of purification methods. Brazilian Journal of Microbiology, São Paulo, v. 46, n. 4, p. 1265-1268, out./dez. 2015. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401265&lng=en&nrm=iso>. Acesso em: 2 abr. 2018. Epub Oct 09, 2015. doi: http://dx.doi.org/10.1590/S1517-838246420140925.pt_BR
dc.identifier.urihttp://repositorio.unb.br/handle/10482/29849-
dc.language.isoenpt_BR
dc.publisherSociedade Brasileira de Microbiologiapt_BR
dc.rightsAcesso Abertopt_BR
dc.titleProduction of Brazilian human norovirus VLPs and comparison of purification methodspt_BR
dc.typeArtigopt_BR
dc.subject.keywordVíruspt_BR
dc.subject.keywordCromatografia de troca iônicapt_BR
dc.rights.licenseBrazilian Journal of Microbiology - All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC-BY (CC BY NC 4.0). Fonte: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401265&lng=en&nrm=iso. Acesso em: 2 abr. 2018.-
dc.identifier.doihttp://dx.doi.org/10.1590/S1517-838246420140925pt_BR
dc.description.abstract1Noroviruses (NVs) are responsible for most cases of human nonbacterial gastroenteritis worldwide. Some parameters for the purification of NV virus-like particles (VLPs) such as ease of production and yield were studied for future development of vaccines and diagnostic tools. In this study, VLPs were produced by the expression of the VP1 and VP2 gene cassette of the Brazilian NV isolate, and two purification methods were compared: cesium chloride (CsCl) gradient centrifugation and ion-exchange chromatography (IEC). IEC produced more and purer VLPs of NV compared to CsCl gradient centrifugation.-
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