DC Field | Value | Language |
dc.contributor.author | Lamounier, Thaís Alves da Costa | pt_BR |
dc.contributor.author | Oliveira, Layssa Miranda de | pt_BR |
dc.contributor.author | Camargo, Brenda Rabello de | pt_BR |
dc.contributor.author | Rodrigues, Kelly Barreto | pt_BR |
dc.contributor.author | Noronha, Eliane Ferreira | pt_BR |
dc.contributor.author | Ribeiro, Bergmann Morais | pt_BR |
dc.contributor.author | Nagata, Tatsuya | pt_BR |
dc.date.accessioned | 2017-12-07T05:13:27Z | - |
dc.date.available | 2017-12-07T05:13:27Z | - |
dc.date.issued | 2015-10 | pt_BR |
dc.identifier.citation | LAMOUNIER, Thais Alves da Costa et al. Production of Brazilian human norovirus VLPs and comparison of purification methods. Brazilian Journal of Microbiology, São Paulo, v. 46, n. 4, p. 1265-1268, out./dez. 2015. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401265&lng=en&nrm=iso>. Acesso em: 2 abr. 2018. Epub Oct 09, 2015. doi: http://dx.doi.org/10.1590/S1517-838246420140925. | pt_BR |
dc.identifier.uri | http://repositorio.unb.br/handle/10482/29849 | - |
dc.language.iso | en | pt_BR |
dc.publisher | Sociedade Brasileira de Microbiologia | pt_BR |
dc.rights | Acesso Aberto | pt_BR |
dc.title | Production of Brazilian human norovirus VLPs and comparison of purification methods | pt_BR |
dc.type | Artigo | pt_BR |
dc.subject.keyword | Vírus | pt_BR |
dc.subject.keyword | Cromatografia de troca iônica | pt_BR |
dc.rights.license | Brazilian Journal of Microbiology - All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC-BY (CC BY NC 4.0). Fonte: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401265&lng=en&nrm=iso. Acesso em: 2 abr. 2018. | - |
dc.identifier.doi | http://dx.doi.org/10.1590/S1517-838246420140925 | pt_BR |
dc.description.abstract1 | Noroviruses (NVs) are responsible for most cases of human nonbacterial gastroenteritis worldwide. Some parameters for the purification of NV virus-like particles (VLPs) such as ease of production and yield were studied for future development of vaccines and diagnostic tools. In this study, VLPs were produced by the expression of the VP1 and VP2 gene cassette of the Brazilian NV isolate, and two purification methods were compared: cesium chloride (CsCl) gradient centrifugation and ion-exchange chromatography (IEC). IEC produced more and purer VLPs of NV compared to CsCl gradient centrifugation. | - |
Appears in Collections: | Artigos publicados em periódicos e afins
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