Título:	Turbo-extraction of glycosides from Stevia rebaudiana using a fractional factorial design
Autor(es):	Martins, Paula M. Lanchote, Aurea D. Thorat, Bhaskar N. Freitas, Luis A.P.
Assunto:	Planejamento experimental
Data de publicação:	Ago-2017
Editora:	Sociedade Brasileira de Farmacognosia
Referência:	MARTINS, Paula M. et al. Turbo-extraction of glycosides from Stevia rebaudiana using a fractional factorial design. Revista Brasileira de Farmacognosia, Curitiba, v. 27, n. 4, p. 510-518, jul./ago. 2017. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2017000400510&lng=en&nrm=iso>. Acesso em: 15 fev. 2018. doi: http://dx.doi.org/10.1016/j.bjp.2017.02.007.
Abstract:	Stevia rebaudiana (Bertoni) Bertoni, Asteraceae, leaf extract has recently called the attention of food industry as a proposal for natural sweetener. The sweet flavor is attributed to the glycosides, in especial stevioside and rebaudioside A, which are the plant main chemical markers. The aim of the work reported here was to optimize the turbo-extraction of stevia leaves using water, ethanol 70% and 90% (w/w) as green solvents. A 25-2 factorial design was applied to study the linear effects of the drug size, solvent to drug ratio, temperature, time and also the turbolysis speed on the extraction of glycosides. The glycosides exhaustive extraction showed that ethanol 70% gave better results and was used for turbo-extraction. The stevioside and rebaudioside A contents were quantified by a validated method by high performance liquid chromatographic with photodiode array detector. The contents of stevioside and rebaudioside A in fluid extract increased with the drug size, but decreased at high shearing speeds and solvent to drug ratio, while their yields decreased at higher temperature and were not affected by turbo speed. An increase in solvent to drug ratio reduced significantly the glycosides percent in dried extract. Optimal solution for S. rebaudiana leaves turbo-extraction was determined by desirability functions. The optimal extraction condition corresponded to drug size of 780 µm, solvent to drug ratio of 10, extraction time of 18 min; temperature of 23 ºC and turbo speed of 20,000 rpm, resulting in yields of 4.98% and 2.70%, for stevioside and rebaudioside A, respectively. These yields are comparable to the ones recently published for dynamic maceration, but with the advantage of shorter extraction times. This work demonstrates that turbolysis is promising for S. rebaudiana glycosides extraction and stimulate new research on the purification of these extracts, which may become an interesting source of income for developing countries such as India and Brazil.
Unidade Acadêmica:	Faculdade UnB Ceilândia (FCE) Curso de Farmácia (FCE-FAR)
Licença:	Revista Brasileira de Farmacognosia - This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivative License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium provided the original work is properly cited and the work is not changed in any way (CC BY NC ND 4.0). Fonte: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2017000400510&lng=en&nrm=iso. Acesso em: 15 fev. 2018.
DOI:	http://dx.doi.org/10.1016/j.bjp.2017.02.007
Aparece nas coleções:	Artigos publicados em periódicos e afins

Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.