Título:	Genome-wide analysis of the transcriptional response to drought stress in root and leaf of common bean
Autor(es):	Pereira, Wendell Jacinto Melo, Arthur Tavares de Oliveira Coelho, Alexandre Siqueira Guedes Rodrigues, Fabiana Aparecida Caldart Mamidi, Sujan Alencar, Sérgio Amorim de Lanna, Anna Cristina Valdisser, Paula Arielle Mendes Ribeiro Brondani, Claudio Nascimento Júnior, Ivanildo Ramalho do Borba, Tereza Cristina de Oliveira Vianello, Rosana Pereira
ORCID:	http://orcid.org/0000-0001-6954-6608 http://orcid.org/0000-0003-0644-5652
Assunto:	Feijão Déficit hídrico Genômica Polimorfismo (Genética)
Data de publicação:	2019
Editora:	Sociedade Brasileira de Genética
Referência:	PEREIRA, Wendell Jacinto et al. Genome-wide analysis of the transcriptional response to drought stress in root and leaf of common bean. Genetics and Molecular Biology, n. 1, e20180259, 2019. DOI: https://doi.org/10.1590/1678-4685-gmb-2018-0259. Disponível em: http://scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019005032102. Acesso em: 01 jul. 2020.
Abstract:	Genes related to the response to drought stress in leaf and root tissue of drought-susceptible (DS) and tolerant (DT) genotypes were characterized by RNA-Seq. In total, 54,750 transcripts, representative of 28,590 genes, were identified; of these, 1,648 were of high-fidelity (merge of 12 libraries) and described for the first time in the Andean germplasm. From the 1,239 differentially expressed genes (DEGs), 458 were identified in DT, with a predominance of genes in categories of oxidative stress, response to stimulus and kinase activity. Most genes related to oxidation-reduction terms in roots were early triggered in DT (T75) compared to DS (T150) suggestive of a mechanism of tolerance by reducing the damage from ROS. Among the KEGG enriched by DEGs up-regulated in DT leaves, two related to the formation of Sulfur-containing compounds, which are known for their involvement in tolerance to abiotic stresses, were common to all treatments. Through qPCR, 88.64% of the DEGs were validated. A total of 151,283 variants were identified and functional effects estimated for 85,780. The raw data files were submitted to the NCBI database. A transcriptome map revealed new genes and isoforms under drought. These results supports a better understanding of the drought tolerance mechanisms in beans.
Licença:	License information: This is an open-access article distributed under the terms of the Creative Commons Attribution License (type CC-BY), which permits unrestricted use, distribution and reproduction in any medium, provided the original article is properly cited.
DOI:	https://doi.org/10.1590/1678-4685-gmb-2018-0259
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