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dc.contributor.authorNery, Flávia M. B.-
dc.contributor.authorMelo, Fernando Lucas-
dc.contributor.authorBoiteux, Leonardo Silva-
dc.contributor.authorRibeiro, Simone G.-
dc.contributor.authorResende, Renato de Oliveira-
dc.contributor.authorOrílio, Anelise Franco-
dc.contributor.authorBatista, Josiane Goulart-
dc.contributor.authorLima, Mirtes Freitas-
dc.contributor.authorCarvalho, Rita de Cássia Pereira-
dc.date.accessioned2020-10-22T13:35:09Z-
dc.date.available2020-10-22T13:35:09Z-
dc.date.issued2020-08-27-
dc.identifier.citationNERY, Flávia M. B. et al. Molecular characterization of Hovenia dulcis-associated virus 1 (HDaV1) and 2 (HDaV2): new tentative species within the order picornavirales. Viruses, v. 12, n. 9, 950, 2020. DOI: https://doi.org/10.3390/v12090950. Disponível em: https://www.mdpi.com/1999-4915/12/9/950. Acesso em: 22 out. 2020.pt_BR
dc.identifier.urihttps://repositorio.unb.br/handle/10482/39576-
dc.language.isoInglêspt_BR
dc.publisherMDPIpt_BR
dc.rightsAcesso Abertopt_BR
dc.titleMolecular characterization of Hovenia dulcis-associated virus 1 (HDaV1) and 2 (HDaV2) : new tentative species within the order picornaviralespt_BR
dc.typeArtigopt_BR
dc.subject.keywordVírus de plantaspt_BR
dc.subject.keywordMetagenômicapt_BR
dc.subject.keywordPicornaviralespt_BR
dc.rights.license© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).pt_BR
dc.identifier.doihttps://doi.org/10.3390/v12090950pt_BR
dc.description.abstract1In a systematic field survey for plant-infecting viruses, leaf tissues were collected from trees showing virus-like symptoms in Brazil. After viral enrichment, total RNA was extracted and sequenced using the MiSeq platform (Illumina). Two nearly full-length picorna-like genomes of 9534 and 8158 nucleotides were found associated with Hovenia dulcis (Rhamnaceae family). Based upon their genomic information, specific primers were synthetized and used in RT-PCR assays to identify plants hosting the viral sequences. The larger contig was tentatively named as Hovenia dulcis-associated virus 1 (HDaV1), and it exhibited low nucleotide and amino acid identities with Picornavirales species. The smaller contig was related to insect-associated members of the Dicistroviridae family but exhibited a distinct genome organization with three non-overlapping open reading frames (ORFs), and it was tentatively named as Hovenia dulcis-associated virus 2 (HDaV2). Phylogenetic analysis using the amino acid sequence of RNA-dependent RNA polymerase (RdRp) revealed that HDaV1 and HDaV2 clustered in distinct groups, and both viruses were tentatively assigned as new members of the order Picornavirales. HDaV2 was assigned as a novel species in the Dicistroviridae family. The 50 ends of both viruses are incomplete. In addition, a nucleotide composition analysis (NCA) revealed that HDaV1 and HDaV2 have similarities with invertebrate-infecting viruses, suggesting that the primary host(s) of these novel virus species remains to be discovered.pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-5791-8002pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-4965-1424pt_BR
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