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dc.contributor.authorAlmeida, Simoneide Souza Titze de-
dc.contributor.authorHorst, Camila Hillesheim-
dc.contributor.authorSánchez, Cristina Soto-
dc.contributor.authorFernandez, Eduardo-
dc.contributor.authorAlmeida, Ricardo Titze de-
dc.date.accessioned2020-11-24T10:53:38Z-
dc.date.available2020-11-24T10:53:38Z-
dc.date.issued2018-07-23-
dc.identifier.citationALMEIDA, Simoneide Souza Titze de et al. Delivery of miRNA-targeted oligonucleotides in the rat striatum by magnetofection with Neuromag®. Molecules, v. 23, n. 7, 1825, 2018. DOI: https://doi.org/10.3390/molecules23071825. Disponível em: https://www.mdpi.com/1420-3049/23/7/1825. Acesso em: 24 nov. 2020.pt_BR
dc.identifier.urihttps://repositorio.unb.br/handle/10482/39653-
dc.language.isoInglêspt_BR
dc.publisherMDPIpt_BR
dc.rightsAcesso Abertopt_BR
dc.titleDelivery of miRNA-targeted oligonucleotides in the rat striatum by magnetofection with Neuromag®pt_BR
dc.typeArtigopt_BR
dc.subject.keywordMicroRNApt_BR
dc.subject.keywordNanopartículaspt_BR
dc.subject.keywordNeuromag®pt_BR
dc.rights.license© 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).pt_BR
dc.identifier.doihttps://doi.org/10.3390/molecules23071825pt_BR
dc.description.abstract1MicroRNAs (miRNAs) regulate gene expression at posttranscriptional level by triggering RNA interference. In such a sense, aberrant expressions of miRNAs play critical roles in the pathogenesis of many disorders, including Parkinson’s disease (PD). Controlling the level of specific miRNAs in the brain is thus a promising therapeutic strategy for neuroprotection. A fundamental need for miRNA regulation (either replacing or inhibition) is a carrier capable of delivering oligonucleotides into brain cells. This study aimed to examine a polymeric magnetic particle, Neuromag®, for delivery of synthetic miRNA inhibitors in the rat central nervous system. We injected the miRNA inhibitor complexed with Neuromag® into the lateral ventricles next to the striatum, by stereotaxic surgery. Neuromag efficiently delivered oligonucleotides in the striatum and septum areas, as shown by microscopy imaging of fluorescein isothiocyanate (FITC)-labeled oligos in astrocytes and neurons. Transfected oligos showed efficacy concerning miRNA inhibition. Neuromag®-structured miR-134 antimiR (0.36 nmol) caused a significant 0.35 fold decrease of striatal miR-134, as revealed by real-time quantitative polymerase chain reaction (RT-qPCR). In conclusion, the polymeric magnetic particle Neuromag® efficiently delivered functional miRNA inhibitors in brain regions surrounding lateral ventricles, particularly the striatum. This delivery system holds potential as a promising miRNA-based disease-modifying drug and merits further pre-clinical studies using animal models of PD.pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-7286-1676pt_BR
dc.description.unidadeFaculdade de Agronomia e Medicina Veterinária (FAV)-
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