Título:	Metabolic activity in cryopreserved and grafted ovarian tissue using high-resolution respirometry
Autor(es):	Rodrigues, Aline de Queiroz Picolo, Victor Luna Goulart, Jair Trapé Silva, Isabella M. G. Ribeiro, Rayane B. Aguiar, Beatriz A. Ferreira, Yasmim B. Oliveira, Daniela Mara de Lucci, Carolina Madeira Bem, Andreza Fabro de Paulini, Fernanda
Assunto:	Metabolismo energético Infecundidade
Data de publicação:	2021
Editora:	Nature
Referência:	RODRIGUES, Aline Q. et al. Metabolic activity in cryopreserved and grafted ovarian tissue using high-resolution respirometry. Scientific Reports, v. 11, 21517, 2021. DOI: https://doi.org/10.1038/s41598-021-01082-z. Disponível em: https://www.nature.com/articles/s41598-021-01082-z. Acesso em: 28 dez. 2021.
Abstract:	Cryopreservation of ovarian tissue followed by transplantation represents a strategy to restore ovarian function and fertility. Stress from cryopreservation-thawing processes can lead to alterations and/or damage to mitochondrial structure and functionality. High resolution respirometry and histological analysis were used to evaluate the effect of cryopreservation and transplantation on ovarian tissue. Four different conditions were performed: Fresh non-transplanted tissue, Fresh transplanted tissue, Cryopreserved non-transplanted tissue and Cryopreserved transplanted tissue. All groups were able to respond to the substrates-uncoupler-inhibitor protocol. We found a dramatic decrease in general oxygen consumption in hemi-ovaries submitted to cryopreservation and/or transplantation. The effect of cryopreservation on mitochondrial metabolism was less intense than effect of transplantation, since the transplantation affected all of the mitochondrial states. A total of 2644 follicles were analyzed. Of these, 2198 were classified as morphologically normal. The percentage of morphologically normal follicles was significantly lower in the Cryopreserved transplanted group when compared to the Cryopreserved non-transplanted group and the Fresh transplanted group (p-value < 0.05). Despite decreased follicular viability and mitochondrial activity, the cryopreservation followed by transplantation of ovarian tissue proved feasible for attempts to restore ovarian function.
Licença:	(CC BY) Open Access Tis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material
DOI:	https://doi.org/10.1038/s41598-021-01082-z
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