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dc.contributor.authorCarvalho, Amandda Évelin Silva de-
dc.contributor.authorSilva, Ingrid Gracielle Martins da-
dc.contributor.authorCorrêa, José Raimundo-
dc.contributor.authorAraujo, Felipe Saldanha de-
dc.date.accessioned2023-09-25T19:38:20Z-
dc.date.available2023-09-25T19:38:20Z-
dc.date.issued2022-11-18-
dc.identifier.citationCARVALHO, Amanda Évelin Silva de et. al. Regulatory T-Cell enhancement, expression of adhesion molecules, and production of anti-inflammatory factors are differentially modulated by spheroid-cultured mesenchymal stem cells. International Journal of Molecular Sciences, [s. l.], v. 23, 14349, 2022. DOI: https://doi.org/10.3390/ijms232214349. Disponível em: https://www.mdpi.com/1422-0067/23/22/14349. Acesso em: 25 set. 2023.pt_BR
dc.identifier.urihttp://repositorio2.unb.br/jspui/handle/10482/46536-
dc.language.isoengpt_BR
dc.publisherMDPIpt_BR
dc.rightsAcesso Abertopt_BR
dc.titleRegulatory T-Cell enhancement, expression of adhesion molecules, and production of anti-inflammatory factors are differentially modulated by spheroid-cultured mesenchymal stem cellspt_BR
dc.typeArtigopt_BR
dc.subject.keywordCélulas-tronco mesenquimaispt_BR
dc.subject.keywordCélulas Tpt_BR
dc.subject.keywordMoléculas de adesão celularpt_BR
dc.rights.license(CC-BY) Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).pt_BR
dc.identifier.doihttps://doi.org/10.3390/ijms232214349pt_BR
dc.description.abstract1The culture of mesenchymal stem cells (MSCs) as spheroids promotes a more physiological cellular behavior, as it more accurately reflects the biological microenvironment. Nevertheless, mixed results have been found regarding the immunosuppressive properties of spheroid-cultured MSCs (3D-MSCs), the mechanisms of immunoregulation of 3D-MSCs being scarcely described at this point. In the present study, we constructed spheroids from MSCs and compared their immunosuppressive potential with that of MSCs cultured in monolayer (2D-MSCs). First, we evaluated the ability of 2D-MSCs and 3D-MSCs to control the activation and proliferation of T-cells. Next, we evaluated the percentage of regulatory T-cells (Tregs) after the co-culturing of peripheral blood mononuclear cells (PBMCs) with 2D-MSCs and 3D-MSCs. Finally, we investigated the expression of adhesion molecules, as well as the expressions of several anti-inflammatory transcripts in 2D-MSCs and 3DMSCs maintained in both inflammatory and non-inflammatory conditions. Interestingly, our data show that several anti-inflammatory genes are up-regulated in 3D-MSCs, and that these cells can control T-cell proliferation. Nevertheless, 2D-MSCs are more efficient in suppressing the immune cell proliferation. Importantly, contrary to what was observed in 3D-MSCs, the expressions of ICAM-1 and VCAM-1 are significantly upregulated in 2D-MSCs exposed to an inflammatory environment. Furthermore, only 2D-MSCs are able to promote the enhancement of Tregs. Taken together, our data clearly show that the immunosuppressive potential of MSCs is significantly impacted by their shape, and highlights the important role of cell–cell adhesion molecules for optimal MSC immunomodulatory function.pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-7654-044Xpt_BR
dc.contributor.affiliationUniversity of Brasília, Hematology and Stem Cells Laboratorypt_BR
dc.contributor.affiliationUniversity of Brasília, Molecular Pharmacology Laboratorypt_BR
dc.contributor.affiliationUniversity of Brasília, Microscopy and Microanalysis Laboratorypt_BR
dc.contributor.affiliationUniversity of Brasília, Microscopy and Microanalysis Laboratorypt_BR
dc.contributor.affiliationUniversity of Brasília, Hematology and Stem Cells Laboratorypt_BR
dc.description.unidadeInstituto de Ciências Biológicas (IB)pt_BR
dc.description.unidadeFaculdade de Ciências da Saúde (FS)pt_BR
dc.description.unidadeDepartamento de Farmácia (FS FAR)pt_BR
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