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dc.contributor.authorJunqueira, Ana Caroline de Oliveira-
dc.contributor.authorMelo, Nadielle Tamires Moreira-
dc.contributor.authorParachin, Nádia Skorupa-
dc.contributor.authorPaes, Hugo Costa-
dc.date.accessioned2023-10-09T15:18:27Z-
dc.date.available2023-10-09T15:18:27Z-
dc.date.issued2023-02-15-
dc.identifier.citationJUNQUEIRA, Ana Caroline de Oliveira et al. Loss of a functional mitochondrial pyruvate carrier in Komagataella phaffii does not improve lactic acid production from glycerol in aerobic cultivation. Microorganisms, [S.l.], v. 11, n. 2, 483, 2023. DOI: https://doi.org/10.3390/microorganisms11020483. Disponível em: https://www.mdpi.com/2076-2607/11/2/483. Acesso em: 09 out. 2023.pt_BR
dc.identifier.urihttp://repositorio2.unb.br/jspui/handle/10482/46641-
dc.language.isoengpt_BR
dc.publisherMDPIpt_BR
dc.rightsAcesso Abertopt_BR
dc.titleLoss of a functional mitochondrial pyruvate carrier in Komagataella phaffii does not improve lactic acid production from glycerol in aerobic cultivationpt_BR
dc.typeArtigopt_BR
dc.subject.keywordTransportador de piruvato mitocondrialpt_BR
dc.subject.keywordHemoglobina bacterianapt_BR
dc.subject.keywordGlicerolpt_BR
dc.rights.licenseCopyright: © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/4.0).pt_BR
dc.identifier.doihttps://doi.org/10.3390/microorganisms11020483pt_BR
dc.description.abstract1Cytosolic pyruvate is an essential metabolite in lactic acid production during microbial fermentation. However, under aerobiosis, pyruvate is transported to the mitochondrial matrix by the mitochondrial pyruvate carrier (MPC) and oxidized in cell respiration. Previous reports using Saccha- romyces cerevisiae or Aspergillus oryzae have shown that the production of pyruvate-derived chemicals is improved by deleting the MPC1 gene. A previous lactate-producing K. phaffii strain engineered by our group was used as a host for the deletion of the MPC1 gene. In addition, the expression of a bacterial hemoglobin gene under the alcohol dehydrogenase 2 promoter from Scheffersomyces stipitis, known to work as a hypoxia sensor, was used to evaluate whether aeration would supply enough oxygen to meet the metabolic needs during lactic acid production. However, unlike S. cerevisiae and A. oryzae, the deletion of Mpc1 had no significant impact on lactic acid production but negatively affected cell growth in K. phaffii strains. Furthermore, the relative quantification of the VHb gene revealed that the expression of hemoglobin was detected even in aerobic cultivation, which indicates that the demand for oxygen in the bioreactor could result in functional hypoxia. Overall, the results add to our previously published ones and show that blocking cell respiration using hypoxia is more suitable than deleting Mpc for producing lactic acid in K. phaffii.pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-0327-232Xpt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-1448-6852pt_BR
dc.contributor.affiliationUniversity of Brasília, Department of Molecular Biologypt_BR
dc.contributor.affiliationCatholic University of Brasília, Genomic Sciences and Biotechnologypt_BR
dc.contributor.affiliationGingko Bioworkspt_BR
dc.contributor.affiliationUniversity of Brasília, University of Brasília Medical School, Clinical Medicine Divisionpt_BR
dc.description.unidadeInstituto de Ciências Biológicas (IB)pt_BR
dc.description.unidadeDepartamento de Biologia Celular (IB CEL)pt_BR
dc.description.unidadeFaculdade de Medicina (FMD)pt_BR
dc.description.ppgPrograma de Pós-Graduação em Biologia Molecularpt_BR
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