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Título: Ramularia leaf spot: PCR‑based methods reveal widespread distribution of Ramulariopsis pseudoglycines and limited presence of R. gossypii in Brazil
Autor(es): Silva, Aline Suelen da
Rennó, Marcelo Henrique Lisboa
Quitania, Ana Clara Ribeiro
Café‑Filho, Adalberto Corrêa
Miller, Robert NeilGerard
Araújo, Alderi Emidio de
Pinho, Danilo Batista
ORCID: https://orcid.org/0000-0002-0550-5847
https://orcid.org/0000-0001-5195-9930
https://orcid.org/0000-0002-8166-7127
https://orcid.org/0000-0002-5204-2961
https://orcid.org/0000-0002-5798-4552
https://orcid.org/0000-0003-0257-1539
https://orcid.org/0000-0003-2624-302X
Afiliação do autor: Universidade de Brasília
Universidade de Brasília
Universidade de Brasília
Universidade de Brasília
Universidade de Brasília
Embrapa Algodão, Campina Grande
Universidade de Brasília
Data de publicação: 17-Jun-2023
Editora: Springer Nature
Referência: SILVA,a line Suelen da et al. Ramularia leaf spot: PCR‑based methods reveal widespread distribution of Ramulariopsis pseudoglycines and limited presence of R. gossypii in Brazil. Scientific Reports, v. 13, 9826, 2023. DOI: https://doi.org/10.1038/s41598-023-33530-3. Disponível em: https://www.nature.com/articles/s41598-023-33530-3. Acesso em: 15 nov. 2023.
Resumo: Whilst Brazil is the fourth largest cotton producer globally, incidence of ramularia leaf spot (RLS) has decreased yield. In 2017–18 and 2018–19, ca. 300 fungal samples were collected throughout Brazil. Hyphal tip cultures were obtained for amplification of the RNA polymerase II (RPB2), 28S rRNA, the ribosomal DNA internal transcribed spacers (ITS), actin (ACT), elongation factor (EF1-α) and histone H3 (HIS3) genomic regions. Additionally, sequences of the glyceraldehyde‑3‑phosphate dehydrogenase (GAPDH) were obtained by nanopore sequencing and the EF1-α region was selected as a marker for rapid recognition of Ramulariopsis species. Clade assignments based on the concatenated‑sequence tree were identical to those in tree generated by RPB2‑sequences, as well as in an RPB2 haplotype network and an ISSR (TGTC) 4 dendrogram, in identification with species‑specific primers and based on morphological comparisons. Out of 267 examined isolates, 252 were identified as Ramulariopsis pseudoglycines, indicating this species as the most widespread causal agent of cotton RLS in the Brazilian growing regions. Species‑specific primers developed in the study that target the EF1-α gene provide an opportunity for extensive RLS sampling worldwide to study the distribution of Ramulariopsis species. Such data will aid breeders and plant pathologists in cotton disease resistance development and fungicide resistance avoidance.
Unidade Acadêmica: Instituto de Ciências Biológicas (IB)
Departamento de Fitopatologia (IB FIT)
Departamento de Biologia Celular (IB CEL)
Licença: Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http:// creativecommons. org/ licenses/ by/4. 0/.
DOI: https://doi.org/10.1038/s41598-023-33530-3
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