| Campo DC | Valor | Idioma |
|---|
| dc.contributor.author | Vieira, Igor Patrick Vasconcelos | - |
| dc.contributor.author | Pimentel, Felipe Seixas Arreguy | - |
| dc.contributor.author | Coelho, Cintia Marques | - |
| dc.contributor.author | De Marco, Janice Lisboa | - |
| dc.contributor.author | Moraes, Lidia Maria Pepe de | - |
| dc.contributor.author | Torres, Fernando Araripe Gonçalves | - |
| dc.date.accessioned | 2024-02-07T14:46:19Z | - |
| dc.date.available | 2024-02-07T14:46:19Z | - |
| dc.date.issued | 2023-11-21 | - |
| dc.identifier.citation | VIEIRA, Igor Patrick Vasconcelos et al. Use of an on/of tetracycline riboswitch to control protein production in Komagataella phafi. AMB Express, v. 13, 131, 2023. DOI: https://doi.org/10.1186/s13568-023-01637-5. Disponível em: https://amb-express.springeropen.com/articles/10.1186/s13568-023-01637-5. Acesso em: 07 fev. 2024. | pt_BR |
| dc.identifier.uri | http://repositorio2.unb.br/jspui/handle/10482/47704 | - |
| dc.language.iso | eng | pt_BR |
| dc.publisher | AMB Express | pt_BR |
| dc.rights | Acesso Aberto | pt_BR |
| dc.title | Use of an on/of tetracycline riboswitch to control protein production in Komagataella phafi | pt_BR |
| dc.type | Artigo | pt_BR |
| dc.subject.keyword | Leveduras | pt_BR |
| dc.subject.keyword | Tetraciclina | pt_BR |
| dc.subject.keyword | Proteínas | pt_BR |
| dc.rights.license | © The Author(s) 2023. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. | pt_BR |
| dc.identifier.doi | https://doi.org/10.1186/s13568-023-01637-5 | pt_BR |
| dc.description.abstract1 | The methylotrophic yeast Komagataella phafi is one of the most important microbial platforms to produce recom binant proteins. Despite its importance in the context of industrial biotechnology, the use of synthetic biology
approaches in K. phafi is hampered by the fact that few genetic tools are available for precise control of gene expres sion in this system. In this work, we used an RNA aptamer activated by tetracycline to modulate protein production
at the translational level. Using lacZ as gene reporter, we have demonstrated signifcant reduction of the heterolo gous protein upon addition of tetracycline. Furthermore, this genetic control device was applied for the control
of Ku70p. This protein is involved in non-homologous recombination and the control of its production paves the way
for the development of strains exhibiting higher rates of homologous recombination. | pt_BR |
| dc.identifier.orcid | https://orcid.org/0000-0002-3650-7271 | pt_BR |
| dc.contributor.affiliation | Universidade de Brasília, Instituto de Ciências Biológicas, Laboratório de Biologia Molecular | pt_BR |
| dc.contributor.affiliation | Universidade Federal do Rio de Janeiro, Instituto de Bioquímica Médica, Laboratório de Biotecnologia de Leveduras | pt_BR |
| dc.contributor.affiliation | Universidade de Brasília, Instituto de Ciências Biológicas, Laboratório de Biologia Sintética | pt_BR |
| dc.contributor.affiliation | Universidade de Brasília, Instituto de Ciências Biológicas, Laboratório de Biologia Molecular | pt_BR |
| dc.contributor.affiliation | Universidade de Brasília, Instituto de Ciências Biológicas, Laboratório de Biologia Molecular | pt_BR |
| dc.contributor.affiliation | Universidade de Brasília, Instituto de Ciências Biológicas, Laboratório de Biologia Molecular | pt_BR |
| dc.description.unidade | Instituto de Ciências Biológicas (IB) | pt_BR |
| dc.description.unidade | Departamento de Biologia Celular (IB CEL) | pt_BR |
| dc.description.unidade | Departamento de Genética e Morfologia (IB GEM) | pt_BR |
| Aparece nas coleções: | Artigos publicados em periódicos e afins
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