Título:	Morphological and ultrastructural study of the palpebral conjunctiva of healthy domestic cats
Autor(es):	Ricci, Cláudia L. Araújo, Rosélia de Lima Sousa Passareli, João V. G. C. Estanho, Giovana J. G. Alves, Débora S. Nai, Gisele A. Andrade, Silvia Franco Corrêa, José Raimundo Galera, Paula Diniz
ORCID:	https://orcid.org/0000-0002-2284-5288 https://orcid.org/0000-0002-6496-2538
Afiliação do autor:	University of Oeste Paulista – UNOESTE, Postgraduate Program in Animal Science University of Brasília, Faculty of Agronomy and Veterinary Medicine UNOESTE, Faculty of Veterinary Medicine UNOESTE, Faculty of Veterinary Medicine UNOESTE, Faculty of Veterinary Medicine UNOESTE, Faculty of Medicine, Department of Pathology UNOESTE, Veterinary Hospital, Department of Veterinary Ophthalmology University of Brasília, Institute of Biological Sciences, Microscopy and Microanalysis Laboratory University of Brasília, Faculty of Agronomy and Veterinary Medicine
Assunto:	Gato Conjutiva palpebral
Data de publicação:	22-Mar-2023
Editora:	Wiley
Referência:	RICCI, Cláudia L. et al. Morphological and ultrastructural study of the palpebral conjunctiva of healthy domestic cats. Veterinary Ophthalmology, [S. l.], v. 26, n. 3, p. 219-224, maio 2023. DOI: https://doi.org/10.1111/vop.13081.
Abstract:	Objective: To describe the morphology of the meibomian glands and goblet cells in the palpebral conjunctiva of healthy cats. Animals studied: Five healthy domestic cats without ocular changes that had died from causes unrelated to the study were evaluated. Procedures: Forty samples were collected from upper and lower palpebral conjunctiva and 20 from palpebral fornix region in the nasal corner. The samples were processed for scanning electron microscopy (SEM), transmission electron microscopy (TEM), and histopathology. Results: In the SEM analysis of the palpebral fornix, numerous points of mucous extrusion between the cell junctions were visualized, along with the presence of microvilli in the apical portions with small secretory vesicles. A homogeneous surface was highlighted, formed by the arrangement of cell contours in the form of hexagons. The grouping of goblet cells and their cytoplasmic vesicles filled with homogeneous content was visualized using TEM. Histopathology showed goblet cells interspersed with stratified epithelium accompanied by well-vascularized connective tissue. In the samples stained with hematoxylin and eosin, the meibomian glands, formed by acinar cells and with the presence of individual openings of the ducts in the eyelid margin, were easily visualized in the eyelid margins. Conclusions: This study describes the ultrastructural form of goblet cells and the morphology of the palpebral conjunctiva of healthy cats by the histopathology of the meibomian glands. This description can serve as a parameter of normality and aid in the detection of morphological alterations in these structures, as well as a parameter for comparison with other animal species.
Unidade Acadêmica:	Faculdade de Agronomia e Medicina Veterinária (FAV) Instituto de Ciências Biológicas (IB) Departamento de Biologia Celular (IB CEL)
DOI:	https://doi.org/10.1111/vop.13081
Versão da editora:	https://onlinelibrary.wiley.com/doi/10.1111/vop.13081
Aparece nas coleções:	Artigos publicados em periódicos e afins

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