Title:	HygR and PurR plasmid vectors for episomal transfection of Trypanosoma cruzi
Authors:	Nóbrega, Otávio de Tolêdo Santana, Jaime Martins de Sturm, Nancy Ritsuko Teixeira, Antonio Raimundo Lima Cruz Campbell, David A.
Assunto::	Tripanossoma cruzi Vetores artrópodes Vetores - controle
Issue Date:	Aug-2004
Publisher:	Instituto Oswaldo Cruz, Ministério da Saúde
Citation:	NOBREGA, Otávio T. et al. HygR and PurR plasmid vectors for episomal transfection of Trypanosoma cruzi. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 99, n. 5, ago. 2004. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000500010&lng=pt&nrm=iso>. Acesso em: 4 dez. 2012. doi: http://dx.doi.org/10.1590/S0074-02762004000500010.
Abstract:	This work describes the development and functional testing of two episomes for stable transfection of Trypanosoma cruzi. pHygD contained the 5'- and 3'- flanking regions of the gene encoding the cathepsin B-like protease of T. cruzi as functional trans-splicing and polyadenylation signals for the hygR ORF. Evidence is presented to support extrachromosomal maintenance and organization as tandem repeats in transfected parasites. pPac was derived from pHygD by replacement of the entire hygR ORF with a purR coding region. The ability to modify pHygD and the availability of the complete DNA sequence make these plasmids useful tools for the genetic manipulation of T. cruzi.
metadata.dc.description.unidade:	Faculdade UnB Ceilândia (FCE) Curso de Saúde Coletiva (FCE-SC)
Licença::	Instituto Oswaldo Cruz, Ministério da Saúde - Todo o conteúdo deste periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons (Attribution-NonCommercial 3.0 Unported (CC BY-NC 3.0)). Fonte: http://www.scielo.br/scielo.php?script=sci_serial&pid=0074-0276&lng=pt&nrm=iso. Acesso em: 4 dez. 2012.
DOI:	http://dx.doi.org/10.1590/S0074-02762004000500010
Appears in Collections:	Artigos publicados em periódicos e afins

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