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Título : Isolation of osteosarcoma-associated human antibodies from a combinatorial fab phage display library
Autor : Barbosa, Carmela Dantas
Faria, Fabrícia Paula de
Brígido, Marcelo de Macedo
Maranhão, Andrea Queiroz
Assunto:: Osteossarcoma
Tumores
Adolescentes
Fecha de publicación : 2009
Editorial : Hindawi Publishing Corporation
Citación : DANTAS-BARBOSA, Carmela et al. Isolation of Osteosarcoma-Associated Human Antibodies from a Combinatorial Fab Phage Display Library. Journal of Biomedicine and Biotechnology, v. 2009, Art. ID 157531, p. 1-8, 2009. Disponível em: <https://www.hindawi.com/journals/bmri/2009/157531/>. Acesso em: 15 dez. 2016.
Resumen : Osteosarcoma, a highly malignant disease, is the most common primary bone tumor and is frequently found in children and adolescents. In order to isolate antibodies against osteosarcoma antigens, a combinatorial osteosarcoma Fab library displayed on the surface of phages was used. After three rounds of selection on the surface of tumor cells, several osteosarcoma-reactive Fabs were detected. From these Fabs, five were better characterized, and despite having differences in their VH (heavy chain variable domain) and (kappa chain variable domain) regions, they all bound to a protein with the same molecular mass. Further analysis by cell ELISA and immunocytochemistry suggested that the Fabs recognize a membrane-associated tumor antigen expressed in higher amounts in neoplasic cells than in normal tissue. These results suggest that the human Fabs selected in this work are a valuable tool for the study of this neoplasia.
Licença:: Copyright © 2009 Carmela Dantas-Barbosa et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Fonte: https://www.hindawi.com/journals/bmri/2009/157531/. Acesso em: 15 dez. 2016.
DOI: https://dx.doi.org/10.1155/2009/157531
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