Título:	Successful isolation of Leishmania infantum from Rhipicephalus sanguineus sensu lato (Acari : Ixodidae) collected from naturally infected dogs
Autor(es):	Silva, Viviane Medeiros Gonçalves, Rodrigo Gurgel Araújo, Nadjar Nitz Silva Lociks de Morales, Lucia Emilia D’ Anduraim Cruz, Laurício Monteiro Sobral, Isabele Gonçalves Boité, Mariana Côrtes Ferreira, Gabriel Eduardo Melim Cupolillo, Elisa Romero, Gustavo Adolfo Sierra
Assunto:	Leishmania Cão Carapato
Data de publicação:	9-Out-2015
Editora:	BioMed Central
Referência:	SILVA, Viviane Medeiros et al. Successful isolation of Leishmania infantum from Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) collected from naturally infected dogs. BMC Veterinary Research, v. 11, Article 258, 9 out. 2015. Disponível em: <https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-015-0576-5>. Acesso em: 14 jun. 2017. doi: https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-015-0576-5.
Abstract:	Background: The main transmission route of Leishmania infantum is through the bites of sand flies. However, alternative mechanisms are being investigated, such as through the bites of ticks, which could have epidemiological relevance. The objective of this work was to verify the presence of Leishmania spp. In Rhipicephalus sanguineus sensu lato collected from naturally infected dogs in the Federal District of Brazil. Methods: Ticks were dissected to remove their intestines and salivary glands for DNA extraction and the subsequent amplification of the conserved region of 120 bp of kDNA and 234 bp of the hsp70 gene of Leishmania spp. The amplified kDNA products were digested with endonucleases HaeIII and BstUI and were submitted to DNA sequencing. Isolated Leishmania parasites from these ticks were analyzed by multilocus enzyme electrophoresis, and the DNA obtained from this culture was subjected to microsatellite analyses. Results: Overall, 130 specimens of R. sanguineus were collected from 27 dogs. Leishmania spp. were successfully isolated in culture from five pools of salivary glands and the intestines of ticks collected from four dogs. The amplified kDNA products from the dog blood samples and from the tick cultures, when digested by HaeIII and BstUI, revealed the presence of L. braziliensis and L. infantum. One strain was cultivated and characterized as L. infantum by enzyme electrophoresis. The amplified kDNA products from the blood of one dog showed a sequence homology with L. braziliensis; however, the amplified kDNA from the ticks collected from this dog showed a sequence homology to L. infantum. Conclusion: The results confirm that the specimens of R. sanguineus that feed on dogs naturally infected by L. infantum contain the parasite DNA in their intestines and salivary glands, and viable L. infantum can be successfully isolated from these ectoparasites.
Unidade Acadêmica:	Faculdade de Medicina (FMD)
Licença:	© 2015 Medeiros-Silva et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
DOI:	https://dx.doi.org/10.1186/s12917-015-0576-5
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