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Titre: Survey of Leptospira spp in pampas deer (Ozotoceros bezoarticus) in the Pantanal wetlands of the state of Mato Grosso do Sul, Brazil by serology and polymerase chain reaction
Auteur(s): Vieira, Anahí Souto
Rosinha, Grácia Maria Soares
Oliveira, Carina Elisei de
Vasconcellos, Silvio Arruda
Lima-Borges, Paulo Andre
Tomás, Walfrido Moraes
Mourão, Guilherme Miranda
Lacerda, Ana Cristina Reis
Soares, Cleber Oliveira
Araújo, Flábio Ribeiro de
Piovezan, Ubiratan
Zucco, Carlos Andre
Pellegrin, Aiesca Oliveira
Assunto:: Pampas deer
Leptospira spp
MAT
PCR
Pantanal
Date de publication: 2011
Editeur: Instituto Oswaldo Cruz, Ministério da Saúde
Référence bibliographique: Mem. Inst. Oswaldo Cruz,v.106,n.6,p.763-768,2011
Résumé: This work reports a survey of Leptospira spp in pampas deer (Ozotoceros bezoarticus) in the Pantanal wetlands of the state of Mato Grosso do Sul, Brazil by serology and polymerase chain reaction (PCR). Seventy pampas deer were captured in the dry season and surveyed using PCR, microscopic agglutination test (MAT) (n = 51) and by both techniques (n = 47). PCR detected infections in two pampas deer and MAT detected infections in three. Through sequencing and phylogenetic analyses, the PCR-amplified fragment detected in deer was identified as Leptospira interrogans. Serovars Pomona and Butembo were detected using MAT and the highest titre was 200 for serovar Pomona. Epidemiological aspects of the findings are discussed.
metadata.dc.description.unidade: Em processamento
DOI: https://dx.doi.org/10.1590/S0074-02762011000600019
Collection(s) :Artigos publicados em periódicos e afins

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