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dc.contributor.authorRôlo, José Luiz Jivago de Paula-
dc.contributor.authorPaulini, F.-
dc.contributor.authorSilva, R. C.-
dc.contributor.authorAraujo, M. S.-
dc.contributor.authorMarinho, A. P. S.-
dc.contributor.authorLucci, C. M.-
dc.date.accessioned2019-01-22T12:30:51Z-
dc.date.available2019-01-22T12:30:51Z-
dc.date.issued2018-
dc.identifier.citationJIVAGO, J. L. P. R. et al. Cryopreservation and characterization of canine preantral follicles. Cryobiology, n. 81, p. 34-42, 2018.pt_BR
dc.identifier.urihttp://repositorio.unb.br/handle/10482/33799-
dc.language.isoInglêspt_BR
dc.publisherElsevierpt_BR
dc.rightsAcesso Abertopt_BR
dc.titleCryopreservation and characterization of canine preantral folliclespt_BR
dc.typeArtigopt_BR
dc.subject.keywordCriopreservação de órgãos, tecidos, etc.pt_BR
dc.subject.keywordCão - reproduçãopt_BR
dc.rights.licenseAutorização concedida ao Repositório Institucional da Universidade de Brasília (RIUnB) pelo servidor José Luiz Jivago de Paula Rôlo, em 15 de janeiro de 2019, para disponibilizar o artigo, gratuitamente, para fins de leitura, impressão e/ou download, a título de divulgação da obra.pt_BR
dc.description.abstract1The aim of this study was to define the population, morphological and ultrastructural characteristics of bitch preantral follicles (PAFs) and to compare the effects on the morphology of PAF of two cryopreservation techniques - slow freezing (SF) and vitrification (V) - of bitches' ovarian tissue. The average population (number per ovary) of PAFs was 48,541 ± 18,366, where 94.25% were primordial (45,145 ± 16,076). The average diameter of the primordial follicles was 27.5 ± 4.2 μm. The overall percentage of morphologically normal PAFs was 93.66 ± 6.81% for the control group, 86.16 ± 11.05% after SF and 68.14 ± 12.75% after V. The percentage of normal primordial follicles was 96.69 ± 4.72% in control, 89.51 ± 10.39% in SF and 75.32 ± 9.23% in V. There was no significant difference in the overall percentage of normal PAFs among SF and the control. However, slow frozen follicles presented ultrastructural damage, while vitrified primordial and primary follicles were well preserved. In conclusion, although slow freezing seemed to be a good preserving method, vitrification was more effective than slow freezing in preserving the ultrastructure of primordial and primary follicles of bitches.pt_BR
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