Title:	Laccase affects the rate of Cryptococcus neoformans nonlytic exocytosis from macrophages
Authors:	Frazão, Stefânia de Oliveira Sousa, Herdson Renney de Silva, Lenise Gonçalves da Folha, Jéssica dos Santos Gorgonha, Kaio César de Melo Oliveira Júnior, Getúlio Pereira de Felipe, Maria Sueli Soares Pereira, Ildinete Silva Casadevall, Arturo Nicola, André Moraes Andrade, Patrícia Albuquerque de
metadata.dc.identifier.orcid:	https://orcid.org/0000-0002-0067-1969 https://orcid.org/0000-0001-7606-0972 https://orcid.org/0000-0001-9861-1042 https://orcid.org/0000-0001-6737-6705 https://orcid.org/0000-0001-6940-401X https://orcid.org/0000-0003-4347-6853 https://orcid.org/0000-0003-1403-9363 https://orcid.org/0000-0002-9402-9167 https://orcid.org/0000-0001-8656-5835 https://orcid.org/0000-0001-8160-7784
Assunto::	Cryptococcus neoformans Leveduras Enzimas de fungos Macrófagos Exocitose não-lítica
Issue Date:	8-Sep-2020
Publisher:	American Society for Microbiology
Citation:	FRAZÃO, Stefânia de Oliveira et al. Laccase affects the rate of Cryptococcus neoformans nonlytic exocytosis from macrophages. mBio, v. 11, n. 5, e02085-20, 2020. DOI: https://doi.org/10.1128/mBio.02085-20. Disponível em: https://mbio.asm.org/content/11/5/e02085-20. Acesso em: 20 jan. 2021.
Abstract:	Nonlytic exocytosis is a process in which previously ingested microbes are expelled from host phagocytes with the concomitant survival of both cell types. This process has been observed in the interaction of Cryptococcus spp. and other fungal cells with phagocytes as distant as mammalian, bird, and fish macrophages and ameboid predators. Despite a great amount of research dedicated to unraveling this process, there are still many questions about its regulation and its final benefits for host or fungal cells. During a study to characterize the virulence attributes of Brazilian clinical isolates of C. neoformans, we observed great variability in their rates of nonlytic exocytosis and noted a correlation between this process and fungal melanin production/laccase activity. Flow cytometry experiments using melanized cells, nonmelanized cells, and lac1Δ mutants revealed that laccase has a role in the process of nonlytic exocytosis that seems to be independent of melanin production. These results identify a role for laccase in virulence, independent of its role in pigment production, that represents a new variable in the regulation of nonlytic exocytosis.
Licença::	Copyright © 2020 Frazão et al. This is an openaccess article distributed under the terms of the Creative Commons Attribution 4.0 International license.
DOI:	https://doi.org/10.1128/mBio.02085-20
Appears in Collections:	Artigos publicados em periódicos e afins UnB - Professores Eméritos

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