Título:	Cytoglobin attenuates neuroinflammation in lipopolysaccharide-activated primary preoptic area cells via NF-κB pathway inhibition
Autor(es):	Gomes, Bruna R. B. Sousa, Gabriela Luna S. de Ott, Daniela Murgott, Jolanta Sousa, Marcelo V. de Souza, Paulo Eduardo Narcizo de Roth, Joachim Souza, Fabiane Hiratsuka Veiga de
Assunto:	Citoglobina Inflamação Febre
Data de publicação:	12-Dez-2019
Editora:	Frontiers
Referência:	GOMES, Bruna R. B. et al. Cytoglobin attenuates neuroinflammation in lipopolysaccharide-activated primary preoptic area cells via NF-κB pathway inhibition. Frontiers in Molecular Neuroscience, v. 12, art. 307, 2019. DOI: https://doi.org/10.3389/fnmol.2019.00307. Disponível em: https://www.frontiersin.org/articles/10.3389/fnmol.2019.00307/full. Acesso em: 02 jun. 2021.
Abstract:	Cytoglobin (Cygb) is a hexacoordinate protein, associated with the transport of oxygen, nitric oxide scavenging, tumor suppression and protection against oxidative stress and inflammation. This protein is expressed in brain areas including the preoptic area (POA) of the anterior hypothalamus, the region responsible for the regulation of body temperature. In this study, we show that Cygb is upregulated in the rat hypothalamus 2.5 h and 5 h after intravenous administration of lipopolysaccharide (LPS). We investigated the effect of treatment with Cygb in POA primary cultures stimulated with LPS for 4 h. The levels of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) were measured and the results showed that Cygb reduced the concentrations of both cytokines. We further observed a decrease in immunoreactivity of the inflammatory transcription factor nuclear factor-κB (NF-κB), but not NF-IL6 and STAT3, in the nucleus of Cygb-treated POA cells. These findings suggest that Cygb attenuates the secretion of IL-6 and TNF-α in LPS-stimulated POA primary cultures via inhibition of the NF-κB signaling pathway, indicating that this protein might play an important role in the control of neuroinflammation and fever.
Unidade Acadêmica:	Faculdade UnB Ceilândia (FCE) Curso de Farmácia (FCE-FAR)
Licença:	Copyright © 2019 Gomes, de Sousa, Ott, Murgott, de Sousa, de Souza, Roth and Veiga-Souza. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
DOI:	https://doi.org/10.3389/fnmol.2019.00307
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