Title:	Oxidonitrergic and antioxidant effects of a low molecular weight peptide fraction from hardened bean (Phaseolus vulgaris) on endothelium
Authors:	Graziane, Daniel Ribeiro, J. V. V. Cruz, V. S. Gomes, R. M. Araujo, E. G. Santos Júnior, Agenor de Castro Moreira dos Tomaz, H. C. M. Castro, C. H. Fontes, Wagner Batista, K. A. Fernandes, K. F. Xavier, C. H.
metadata.dc.identifier.orcid:	http://orcid.org/0000-0002-4997-2658 http://orcid.org/0000-0003-3937-1071 http://orcid.org/0000-0002-8914-5964 http://orcid.org/0000-0002-9012-3287 http://orcid.org/0000-0002-0242-2827 http://orcid.org/0000-0001-8485-2603 http://orcid.org/0000-0003-2079-2140 http://orcid.org/0000-0002-9510-1124 http://orcid.org/0000-0001-5140-8573 http://orcid.org/0000-0003-4396-032X http://orcid.org/0000-0002-6558-5447 http://orcid.org/0000-0003-4006-8213
Assunto::	Feijão Peptídeos Stress oxidativo Óxido nítrico
Issue Date:	2021
Publisher:	Associação Brasileira de Divulgação Científica
Citation:	GRAZIANI, D. et al. Oxidonitrergic and antioxidant effects of a low molecular weight peptide fraction from hardened bean (Phaseolus vulgaris) on endothelium. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 54, n. 6, e10423, 2021. DOI: https://doi.org/10.1590/1414-431x202010423. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021000600602&lng=en&nrm=iso. Acesso em: 13 maio 2021.
Abstract:	About 3000 tons of beans are not used in human food due to hardening. Several studies on bean-derived bioactive peptides have shown potential to treat some diseases, including those relying on oxidative dysfunctions. We assessed the effects of peptides extracted from hardened bean Phaseolus vulgaris (PV) on reactive oxygen species (ROS) and nitric oxide (NO) production, cytotoxic and cytoprotective effects in endothelial cells, and oxidonitrergic-dependent vasodilating effects. Extract was composed by peptide fraction <3 kDa (PV3) from hardened common bean residue. PV3 sequences were obtained and analyzed with bioinformatics. Human umbilical vein endothelial cells were treated with 10, 20, 30, and 250 µg/mL PV3. Oxidative stress was provoked by 3% H2O2. Cytotoxicity and cytoprotective effects were evaluated by MTT assay, whereas, ROS and NO were quantified using DHE and DAF-FM fluorescent probes by confocal microscopy. NO- and endothelium-dependent vasodilating effects of PV3 were assessed in isolated aortic rings. We found 35 peptides with an average mass of 1.14 kDa. There were no cell deaths with 10 and 20 μg/mL PV3. PV3 at 30 μg/mL increased cell viability, while cytotoxicity was observed only with 250 μg/mL PV3. PV3 at 10 μg/mL was able to protect cells from oxidative stress. PV3 also increased NO release without causing cell death. It also reduced relative ROS production induced by H2O2. PV3 vasodilating effects relied on endothelium-dependent NO release. PV3 obtained from low-commercial-value bean displays little cytotoxicity and exerts antioxidant effects, whereas it increases endothelial NO release.
Licença::	(CC BY)
DOI:	https://doi.org/10.1590/1414-431X202010423
Appears in Collections:	Artigos publicados em periódicos e afins

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