Título:	Periphyton diversity in two different Antarctic lakes assessed using metabarcoding
Autor(es):	Câmara, Paulo Eduardo Aguiar Saraiva Souza, Láuren M. D. de Pinto, Otávio Henrique Bezerra Convey, Peter Amorim, Eduardo T. Silva, Micheline Carvalho Rosa, Luiz Henrique
ORCID:	https://orcid.org/0000-0002-3944-996X https://orcid.org/0000-0001-6007-3840 https://orcid.org/0000-0002-8382-2987 https://orcid.org/0000-0001-8497-9903 https://orcid.org/0000-0003-4253-1339 https://orcid.org/0000-0002-2389-3804 https://orcid.org/0000-0002-2389-3804 https://orcid.org/0000-0001-9749-5182
Assunto:	Antártica Sequenciamento de alto rendimento Espaçador ITS
Data de publicação:	2021
Editora:	Springer
Referência:	CÂMARA, Paulo E. A. S. et al. Periphyton diversity in two different Antarctic lakes assessed using metabarcoding. Antarctic Science, v. 33, n. 6, p. 596-604, 2021. DOI: https://doi.org/10.1017/S0954102021000316. Disponível em: https://www.cambridge.org/core/journals/antarctic-science/article/periphyton-diversity-in-two-different-antarctic-lakes-assessed-using-metabarcoding/969D76E3970A1DAD4E54097F9165DA0E. Acesso em: 26 abr. 2022.
Abstract:	Antarctic lakes have generally simple periphyton communities when compared with those of lower latitudes. To date, assessment of microbial diversity in Antarctica has relied heavily on traditional direct observation and cultivation methods. In this study, sterilized cotton baits were left submerged for two years in two lakes on King George Island and Deception Island, South Shetland Islands (Maritime Antarctic), followed by assessment of diversity by metabarcoding using high-throughput sequencing. DNA sequences of 44 taxa belonging to four kingdoms and seven phyla were found. Thirty-six taxa were detected in Hennequin Lake on King George Island and 20 taxa were detected in Soto Lake on Deception Island. However, no significant difference in species composition was detected between the two assemblages (Shannon index). Our data suggest that metabarcoding provides a suitable method for the assessment of periphyton biodiversity in oligotrophic Antarctic lakes.
Licença:	Copyright © Antarctic Science Ltd 2021
DOI:	https://doi.org/10.1017/S0954102021000316
Versão da editora:	https://www.cambridge.org/core/journals/antarctic-science/article/periphyton-diversity-in-two-different-antarctic-lakes-assessed-using-metabarcoding/969D76E3970A1DAD4E54097F9165DA0E
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