Título :	In vitro evaluation of NLS-DTX activity in triple-negative breast cancer
Autor :	Paiva, Karen Letycia Rodrigues de Radicchi, Marina A. Báo, Sônia Nair
metadata.dc.contributor.email:	mailto:karendepaiva@gmail.com mailto:maradicchi.bep@gmail.com mailto:snbao@unb.br
metadata.dc.identifier.orcid:	https://orcid.org/0000-0001-9149-316X https://orcid.org/0000-0002-9873-3098
Assunto::	Nanopartículas lipídicas sólidas Nanopartículas Câncer de mama triplo negativo Docetaxel Mamas - câncer
Fecha de publicación :	2-ago-2022
Editorial :	MDPI
Citación :	PAIVA, Karen L. R.; RADICCHI, Marina A.; BÁO, Sônia N. In vitro evaluation of NLS-DTX activity in triple-negative breast cancer. Molecules , Basel, v. 27, n. 15, art. 4920, 2022. DOI 10.3390/molecules27154920. Disponível em: https://www.mdpi.com/1420-3049/27/15/4920. Acesso em: 12 set. 2022.
Abstract:	Cancer is one of the most lethal diseases in the world, and the development and improvement of treatments used in cancer therapies are extremely important for a better quality of life for patients. In view of the current problems in drug administration such as low solubility and adverse effects, the activity of a solid lipid nanoparticle containing docetaxel (SLN-DTX), a drug already used in conventional therapies, was evaluated in a cell line (MDA-MB-231) of one of the most aggressive types of breast cancer with the worst prognosis, triple-negative breast cancer. Viability tests indicated that SLN-DTX has a greater dependence on the treatment dose when compared to the free drug, which indicates a more controlled release of the drug, and both reduced viability by around 50% at a concentration of 1 µg/mL after 72 h. Transmission electron microscopy (TEM) and confocal and light microscopy analyses indicated that after treatment the cells enter a mitotic catastrophe, characteristic of antimitotic drugs that usually make cells progress to death or senescence. Cells treated with both DTX and SLN-DTX showed significant inhibition of mobility, 73.6% and 66.5% when treated with SLN-DTX and DTX, respectively, compared to the 11.4% of the control after 72 h, characteristics that are very relevant in tumor development and progression. SLN-DTX demonstrated its great potential as a nanocarrier by maintaining and improving the drug’s action in the MDA-MB-231 cell line.
Licença::	Molecules - No special permission is required to reuse all or part of article published by MDPI, including figures and tables. For articles published under an open access Creative Common CC BY license, any part of the article may be reused without permission provided that the original article is clearly cited. Reuse of an article does not imply endorsement by the authors or MDPI. Fonte: https://www.mdpi.com/openaccess. Acesso em: 12 set. 2022.
DOI:	https://doi.org/10.3390/molecules27154920
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