RGBradford : accurate measurement of protein concentration using a smartphone camera and the blue to green intensity ratio

Moreira, Daniel Carneiro

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Campo DC	Valor	Idioma
dc.contributor.author	Moreira, Daniel Carneiro	-
dc.date.accessioned	2022-09-15T21:55:09Z	-
dc.date.available	2022-09-15T21:55:09Z	-
dc.date.issued	2022	-
dc.identifier.citation	MOREIRA, Daniel C. RGBradford: accurate measurement of protein concentration using a smartphone camera and the blue to green intensity ratio. Analytical Biochemistry, v. 655, art. 114839, 15 October 2022. DOI 10.1016/j.ab.2022.114839. Disponível em: https://www.sciencedirect.com/science/article/pii/S0003269722002998?via%3Dihub. Acesso em: 15 set. 2022.	pt_BR
dc.identifier.uri	https://repositorio.unb.br/handle/10482/44835	-
dc.language.iso	Inglês	pt_BR
dc.publisher	Science Direct	pt_BR
dc.rights	Acesso Restrito	pt_BR
dc.title	RGBradford : accurate measurement of protein concentration using a smartphone camera and the blue to green intensity ratio	pt_BR
dc.type	Artigo	pt_BR
dc.subject.keyword	Colorimetria	pt_BR
dc.subject.keyword	Espectrofotometria	pt_BR
dc.identifier.doi	https://doi.org/10.1016/j.ab.2022.114839	pt_BR
dc.relation.publisherversion	https://www.sciencedirect.com/science/article/pii/S0003269722002998?via%3Dihub	pt_BR
dc.description.abstract1	The color shift caused by the interaction between proteins and Coomassie Brilliant Blue in the Bradford assay can be recorded with a smartphone camera. Color data can then be extracted from pictures and processed as analytical signals. Here, I present a simple procedure to accurately measure protein levels using color data from pictures of microplates. Plotting the ratio of blue to green intensity (RGB scale) as a function of protein con centration results in a linear (R2 ≥0.99) relationship, from which protein levels in biological samples can be calculated with no significant difference from values obtained using absorbance data (RGB versus absorbance curves).	pt_BR
dc.identifier.orcid	https://orcid.org/0000-0003-1961-7281	pt_BR
dc.contributor.email	mailto: moreiradc@unb.br	pt_BR
dc.description.unidade	Faculdade de Medicina (FM)	-
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