Titre:	RGBradford : accurate measurement of protein concentration using a smartphone camera and the blue to green intensity ratio
Auteur(s):	Moreira, Daniel Carneiro
metadata.dc.contributor.email:	mailto: moreiradc@unb.br
metadata.dc.identifier.orcid:	https://orcid.org/0000-0003-1961-7281
Assunto::	Colorimetria Espectrofotometria
Date de publication:	2022
Editeur:	Science Direct
Référence bibliographique:	MOREIRA, Daniel C. RGBradford: accurate measurement of protein concentration using a smartphone camera and the blue to green intensity ratio. Analytical Biochemistry, v. 655, art. 114839, 15 October 2022. DOI 10.1016/j.ab.2022.114839. Disponível em: https://www.sciencedirect.com/science/article/pii/S0003269722002998?via%3Dihub. Acesso em: 15 set. 2022.
Abstract:	The color shift caused by the interaction between proteins and Coomassie Brilliant Blue in the Bradford assay can be recorded with a smartphone camera. Color data can then be extracted from pictures and processed as analytical signals. Here, I present a simple procedure to accurately measure protein levels using color data from pictures of microplates. Plotting the ratio of blue to green intensity (RGB scale) as a function of protein con centration results in a linear (R2 ≥0.99) relationship, from which protein levels in biological samples can be calculated with no significant difference from values obtained using absorbance data (RGB versus absorbance curves).
metadata.dc.description.unidade:	Faculdade de Medicina (FM)
DOI:	https://doi.org/10.1016/j.ab.2022.114839
metadata.dc.relation.publisherversion:	https://www.sciencedirect.com/science/article/pii/S0003269722002998?via%3Dihub
Collection(s) :	Artigos publicados em periódicos e afins

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