Title:	Parasite persistence in treated chagasic patients revealed by xenodiagnosis and polymerase chain reaction
Authors:	Britto, Constança Silveira, Celeste Aída Nogueira Cardoso, Maria Angelica Marques, Patricia Ostermayer, Alejandro Luquetti Macêdo, Vanize de Oliveira Fernandes, Octavio
Assunto::	Trypanosoma cruzi DNA de cinetoplasto Chagas, Doença de PCR Tratamento
Issue Date:	Aug-2001
Publisher:	Instituto Oswaldo Cruz, Ministério da Saúde
Citation:	BRITTO, Constança et al. Parasite persistence in treated chagasic patients revealed by xenodiagnosis and polymerase chain reaction. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 96, n. 6, p. 823-826, ago. 2001. DOI: https://doi.org/10.1590/S0074-02762001000600014. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000600014&lng=en&nrm=iso. Acesso em: 19 mar. 2021.
Abstract:	Polymerase chain reaction (PCR) was compared with xenodiagnosis performed 20 years after trypanocidal chemotherapy to investigate parasite clearance. Eighty-five seropositive individuals for Chagas disease presenting a positive xenodiagnosis were treated with specific drugs; 37 in the acute phase and 48 in the chronic phase. Fifteen chronic assymptomatic patients received a placebo. Treatment in the acute phase led to PCR negative results in 73% of the cases, while xenodiagnosis was negative in 86%. In the chronic phase, PCR was negative in 65% of the patients and 83% led to xenodiagnosis negative results. Regarding the untreated group (placebo), 73% gave negative results by xenodiagnosis, of which 36% were positive by PCR. Individuals that were considered seronegative (n=10), presented unequivocally negative results in the PCR demonstrating the elimination of parasite DNA. Seventeen individuals had their antibodies titers decreased to such a level that the final results were considered as doubtful and 16 of them presented negative PCR. The molecular method represents a clear advantage over conventional techniques to demonstrate persistent infections in Chagas disease patients that underwent chemotherapy.
metadata.dc.description.unidade:	Faculdade de Medicina (FMD)
Licença::	Memórias do Instituto Oswaldo Cruz - All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License (CC BY NC 4.0). Fonte: https://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000600014&lng=en&tlng=en. Acesso em: 19 mar. 2021.
DOI:	https://dx.doi.org/10.1590/S0074-02762001000600014
Appears in Collections:	Artigos publicados em periódicos e afins

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