Title:	Accuracy of mucocutaneous leishmaniasis diagnosis using polymerase chain reaction : systematic literature review and meta-analysis
Authors:	Gomes, Ciro Martins Mazin, Suleimy Cristina Santos, Elisa Raphael dos Cesetti, Mariana Vicente Bächtold, Guilherme Albergaria Brízida Cordeiro, João Henrique de Freitas Theodoro, Fabrício Claudino Estrela Terra Damasco, Fabiana dos Santos Carranza, Sebastián Andrés Vernal Santos, Adriana de Oliveira Roselino, Ana Maria Sampaio, Raimunda Nonata Ribeiro
Assunto::	Leishmaniose Diagnóstico
Issue Date:	Apr-2015
Publisher:	Instituto Oswaldo Cruz, Ministério da Saúde
Citation:	GOMES, Ciro Martins et al. Accuracy of mucocutaneous leishmaniasis diagnosis using polymerase chain reaction: systematic literature review and meta-analysis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 110, n. 2, p. 157-165, abr. 2015. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000200157&lng=en&nrm=iso>. Acesso em: 9 maio 2018. Epub Mar 31, 2015. doi: http://dx.doi.org/10.1590/0074-02760140280.
Abstract:	The diagnosis of mucocutaneous leishmaniasis (MCL) is hampered by the absence of a gold standard. An accurate diagnosis is essential because of the high toxicity of the medications for the disease. This study aimed to assess the ability of polymerase chain reaction (PCR) to identify MCL and to compare these results with clinical research recently published by the authors. A systematic literature review based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses: the PRISMA Statement was performed using comprehensive search criteria and communication with the authors. A meta-analysis considering the estimates of the univariate and bivariate models was performed. Specificity near 100% was common among the papers. The primary reason for accuracy differences was sensitivity. The meta-analysis, which was only possible for PCR samples of lesion fragments, revealed a sensitivity of 71% [95% confidence interval (CI) = 0.59; 0.81] and a specificity of 93% (95% CI = 0.83; 0.98) in the bivariate model. The search for measures that could increase the sensitivity of PCR should be encouraged. The quality of the collected material and the optimisation of the amplification of genetic material should be prioritised.
metadata.dc.description.unidade:	Faculdade de Medicina (FMD)
Licença::	Memórias do Instituto Oswaldo Cruz - All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License (CC BY NC 4.0). Fonte: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000200157&lng=en&nrm=iso. Acesso em: 9 maio 2018.
DOI:	http://dx.doi.org/10.1590/0074-02760140280
Appears in Collections:	Artigos publicados em periódicos e afins

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