Título :	Mesenchymal stem cell-like properties of CD133+ glioblastoma initiating cells
Autor :	Pavon, Lorena Favaro Sibov, Tatiana Tais Oliveira, Daniela Mara de Marti, Luciana C. Cabral, Francisco Romero Souza, Jean Gabriel de Boufleur, Pamela Malheiros, Suzana M. F. Neto, Manuel A. de Paiva Cruz, Edgard Ferreira da Tavassi, Ana Marisa Chudzinski Cavalheiro, Sérgio
Assunto::	Neurosferas Glicoproteínas Células-tronco Microscopia eletrônica Tumores
Fecha de publicación :	may-2016
Editorial :	Impact Journals
Citación :	PAVON, Lorena Favaro et al. Mesenchymal stem cell-like properties of CD133+ glioblastoma initiating cells. Oncotarget, Nova York, v. 7, p. 40546-40557, maio 2016. DOI: https://doi.org/10.18632/oncotarget.9658. Disponível em: http://www.impactjournals.com/oncotarget/index.php?journal=oncotarget&page=article&op=view&path[]=9658&path[]=41659. Acesso em: 11 dez. 2018.
Abstract:	Glioblastoma is composed of dividing tumor cells, stromal cells and tumor initiating CD133+ cells. Recent reports have discussed the origin of the glioblastoma CD133+ cells and their function in the tumor microenvironment. The present work sought to investigate the multipotent and mesenchymal properties of primary highly purified human CD133+ glioblastoma-initiating cells. To accomplish this aim, we used the following approaches: i) generation of tumor subspheres of CD133+ selected cells from primary cell cultures of glioblastoma; ii) analysis of the expression of pluripotency stem cell markers and mesenchymal stem cell (MSC) markers in the CD133+ glioblastoma-initiating cells; iii) side-by-side ultrastructural characterization of the CD133+ glioblastoma cells, MSC and CD133+ hematopoietic stem cells isolated from human umbilical cord blood (UCB); iv) assessment of adipogenic differentiation of CD133+ glioblastoma cells to test their MSC-like in vitro differentiation ability; and v) use of an orthotopic glioblastoma xenograft model in the absence of immune suppression. We found that the CD133+ glioblastoma cells expressed both the pluripotency stem cell markers (Nanog, Mush-1 and SSEA-3) and MSC markers. In addition, the CD133+ cells were able to differentiate into adipocyte-like cells. Transmission electron microscopy (TEM) demonstrated that the CD133+ glioblastoma-initiating cells had ultrastructural features similar to those of undifferentiated MSCs. In addition, when administered in vivo to non-immunocompromised animals, the CD133+ cells were also able to mimic the phenotype of the original patient’s tumor. In summary, we showed that the CD133+ glioblastoma cells express molecular signatures of MSCs, neural stem cells and pluripotent stem cells, thus possibly enabling differentiation into both neural and mesodermal cell types.
Licença::	Oncotarget - All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License (CC BY). Fonte: http://www.oncotarget.com/index.php?journal=oncotarget&page=article&op=view&path[]=9658&path[]=41659. Acesso em: 11 dez. 2018.
DOI:	https://doi.org/10.18632/oncotarget.9658
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